Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells
Identifieur interne : 002D09 ( Main/Exploration ); précédent : 002D08; suivant : 002D10Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells
Auteurs : Patrick Midoux [France] ; Christina Mendes ; Alain Legrand [France] ; Jacques Raimond ; Roger Mayer [France] ; Michel Monsigny [France] ; Annie Claude Roche [France]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 1993-02-25.
Abstract
Plasmid DNA/glycosylated polylysine complexes were used to transfer in vitro a luciferase reporter gene into human hepatoma cells by a receptor-mediated endocytosis process. HepG2 cells which express a galactose specific membrane lectin were efficiently and selectively transfected with pSV2Luc/lactosylated polylysine complexes in a sugar dependent manner: i) HepG2 cells which do not express membrane lectin specific for mannose were quite poorly transfected with pSV2Luc/mannosylated polylysine complexes, ii) HeLa cells which do not express membrane lectin specific for galactose were not transfected with pSV2Luc/lactosylated polylysine complexes. The transfection efficiency of HepG2 cells with pSV2Luc/lactosylated polylysine complexes was greatly enhanced either in the presence of chloroquine or in the presence of a fusogenic peptide. A 22-residue peptide derived from the influenza virus hemagglutinin HA2 N-terminal polypeptide that mimics the fusogenic activity of the virus, was selected. In the presence of the fusogenic peptide, the luciferase activity in HepG2 cells was 10 fold larger than that of cells transfected with pSV2Luc/lactosylated polylysine complexes in the presence of chloroquine.
Url:
DOI: 10.1093/nar/21.4.871
Affiliations:
- France
- Centre-Val de Loire, Région Centre
- Orléans
- Centre Val de Loire Université, Université d'Orléans
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: 000219
- to stream Hal, to step Curation: 000219
- to stream Hal, to step Checkpoint: 000259
- to stream Main, to step Merge: 002D71
- to stream Main, to step Curation: 002D09
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells</title>
<author><name sortKey="Midoux, Patrick" sort="Midoux, Patrick" uniqKey="Midoux P" first="Patrick" last="Midoux">Patrick Midoux</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
<author><name sortKey="Mendes, Christina" sort="Mendes, Christina" uniqKey="Mendes C" first="Christina" last="Mendes">Christina Mendes</name>
</author>
<author><name sortKey="Legrand, Alain" sort="Legrand, Alain" uniqKey="Legrand A" first="Alain" last="Legrand">Alain Legrand</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
<author><name sortKey="Raimond, Jacques" sort="Raimond, Jacques" uniqKey="Raimond J" first="Jacques" last="Raimond">Jacques Raimond</name>
</author>
<author><name sortKey="Mayer, Roger" sort="Mayer, Roger" uniqKey="Mayer R" first="Roger" last="Mayer">Roger Mayer</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
<author><name sortKey="Monsigny, Michel" sort="Monsigny, Michel" uniqKey="Monsigny M" first="Michel" last="Monsigny">Michel Monsigny</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
<author><name sortKey="Roche, Annie Claude" sort="Roche, Annie Claude" uniqKey="Roche A" first="Annie Claude" last="Roche">Annie Claude Roche</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-02138456</idno>
<idno type="halId">hal-02138456</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-02138456</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-02138456</idno>
<idno type="doi">10.1093/nar/21.4.871</idno>
<date when="1993-02-25">1993-02-25</date>
<idno type="wicri:Area/Hal/Corpus">000219</idno>
<idno type="wicri:Area/Hal/Curation">000219</idno>
<idno type="wicri:Area/Hal/Checkpoint">000259</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000259</idno>
<idno type="wicri:doubleKey">0305-1048:1993:Midoux P:specific:gene:transfer</idno>
<idno type="wicri:Area/Main/Merge">002D71</idno>
<idno type="wicri:Area/Main/Curation">002D09</idno>
<idno type="wicri:Area/Main/Exploration">002D09</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells</title>
<author><name sortKey="Midoux, Patrick" sort="Midoux, Patrick" uniqKey="Midoux P" first="Patrick" last="Midoux">Patrick Midoux</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
<author><name sortKey="Mendes, Christina" sort="Mendes, Christina" uniqKey="Mendes C" first="Christina" last="Mendes">Christina Mendes</name>
</author>
<author><name sortKey="Legrand, Alain" sort="Legrand, Alain" uniqKey="Legrand A" first="Alain" last="Legrand">Alain Legrand</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
<author><name sortKey="Raimond, Jacques" sort="Raimond, Jacques" uniqKey="Raimond J" first="Jacques" last="Raimond">Jacques Raimond</name>
</author>
<author><name sortKey="Mayer, Roger" sort="Mayer, Roger" uniqKey="Mayer R" first="Roger" last="Mayer">Roger Mayer</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
<author><name sortKey="Monsigny, Michel" sort="Monsigny, Michel" uniqKey="Monsigny M" first="Michel" last="Monsigny">Michel Monsigny</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
<author><name sortKey="Roche, Annie Claude" sort="Roche, Annie Claude" uniqKey="Roche A" first="Annie Claude" last="Roche">Annie Claude Roche</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno>
<idno type="RNSR">196717612S</idno>
<orgName>Centre de biophysique moléculaire</orgName>
<orgName type="acronym">CBM</orgName>
<date type="start">1967-01-01</date>
<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://cbm.cnrs-orleans.fr/</ref>
</desc>
<listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation>
<relation name="UPR4301" active="#struct-303623" type="direct"></relation>
<relation name="UPR4301" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno>
<idno type="ISNI">0000000121581666 </idno>
<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-orleans.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Orléans</settlement>
<region type="old region" nuts="2">Région Centre</region>
<region type="region" nuts="2">Centre-Val de Loire</region>
</placeName>
<orgName type="university">Université d'Orléans</orgName>
<orgName type="institution" wicri:auto="newGroup">Centre Val de Loire Université</orgName>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1093/nar/21.4.871</idno>
<series><title level="j">Nucleic Acids Research</title>
<idno type="ISSN">0305-1048</idno>
<imprint><date type="datePub">1993-02-25</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"> <p>Plasmid DNA/glycosylated polylysine complexes were used to transfer in vitro a luciferase reporter gene into human hepatoma cells by a receptor-mediated endocytosis process. HepG2 cells which express a galactose specific membrane lectin were efficiently and selectively transfected with pSV2Luc/lactosylated polylysine complexes in a sugar dependent manner: i) HepG2 cells which do not express membrane lectin specific for mannose were quite poorly transfected with pSV2Luc/mannosylated polylysine complexes, ii) HeLa cells which do not express membrane lectin specific for galactose were not transfected with pSV2Luc/lactosylated polylysine complexes. The transfection efficiency of HepG2 cells with pSV2Luc/lactosylated polylysine complexes was greatly enhanced either in the presence of chloroquine or in the presence of a fusogenic peptide. A 22-residue peptide derived from the influenza virus hemagglutinin HA2 N-terminal polypeptide that mimics the fusogenic activity of the virus, was selected. In the presence of the fusogenic peptide, the luciferase activity in HepG2 cells was 10 fold larger than that of cells transfected with pSV2Luc/lactosylated polylysine complexes in the presence of chloroquine.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Centre-Val de Loire</li>
<li>Région Centre</li>
</region>
<settlement><li>Orléans</li>
</settlement>
<orgName><li>Centre Val de Loire Université</li>
<li>Université d'Orléans</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Mendes, Christina" sort="Mendes, Christina" uniqKey="Mendes C" first="Christina" last="Mendes">Christina Mendes</name>
<name sortKey="Raimond, Jacques" sort="Raimond, Jacques" uniqKey="Raimond J" first="Jacques" last="Raimond">Jacques Raimond</name>
</noCountry>
<country name="France"><region name="Région Centre"><name sortKey="Midoux, Patrick" sort="Midoux, Patrick" uniqKey="Midoux P" first="Patrick" last="Midoux">Patrick Midoux</name>
</region>
<name sortKey="Legrand, Alain" sort="Legrand, Alain" uniqKey="Legrand A" first="Alain" last="Legrand">Alain Legrand</name>
<name sortKey="Mayer, Roger" sort="Mayer, Roger" uniqKey="Mayer R" first="Roger" last="Mayer">Roger Mayer</name>
<name sortKey="Monsigny, Michel" sort="Monsigny, Michel" uniqKey="Monsigny M" first="Michel" last="Monsigny">Michel Monsigny</name>
<name sortKey="Roche, Annie Claude" sort="Roche, Annie Claude" uniqKey="Roche A" first="Annie Claude" last="Roche">Annie Claude Roche</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002D09 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002D09 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= ChloroquineV1 |flux= Main |étape= Exploration |type= RBID |clé= Hal:hal-02138456 |texte= Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells }}
This area was generated with Dilib version V0.6.33. |